The function of catecholase Essay Example for Free

The function of catecholase Essay5 mL of enzyme, and all of the trials were set up as two tubes to begin with. One tube had 3. 5 mL of urine and 2. 0 mL of catechol, and the other had . 5 mL of enzyme. By charge the enzyme and substrate separate, we were able to have more control over the starting point of the reaction, and were able to bring each individual component to the desired temperature ahead starting. The temperatures that our group tested were 37oC, 45oC, 50oC, 55oC, 65oC, and 75oC. Both our group and group A-4 tested 37 and 45 degrees. This allowed us to compare each groups results more accurately.The setup for the actual tense out was that each temperature had its own test tube rack. Four tubes of water and catechol and four tubes of enzyme were put into each rack, and these racks were wherefore submerged into either a hot water bath or a circulating water bath, depending on which temperature was being tested. The tubes were allowed to sit in the water for appr oximately v minutes to allow the circumscribe of the tubes to come up to the temperature we were trying to test. Then the tube of enzyme was poured into the tube of catechol, the tube was covered and inverted to mix the contents, and the timer was started.We allowed the reaction to run from three to five minutes, keeping track of on the dot how long each set went for. Our method for ensuring that the reactions ran for the exact length of time stated was to start each reaction 30 seconds later than the first, and then take the measurements from the spectrophotometer exactly thirty seconds apart, keeping the tubes in the selfsame(prenominal) order. Before measuring each tube, we again inverted it several clock to make sure that the product was uniformly distributed throughout the tube. Results Results for Group A-5 Temperature (degrees Celsius) Trial 1.Trial 2 Trial 3 Trial 4 Mean stock Deviation 3 (Table 2) (Graph 1 (Graph 2) accord to our results, the optimal temperature for the reaction of catecholase is 45oC. We were able to determine this because the average reaction rate was highest at this temperature (see table 1). We were go down in presumptuous that the reaction would gradually speed up as the temperature increased until it reached a point where the enzyme began to denature, and then the reaction rate would slow after that. This gradual increase, peak, and then decrease can be seen in the graph 1 above. The bars above and below each data point are representations of the standard divergency associated with each value.This shows how much variation we had within each set of trials. When we compared our results to group A-4, it was clear that they too found the peak reaction rate to be at the 45-degree temperature. This served to further verify our results. It is interesting to note that in the graph of both of our data, although the actual values are not the same, the overall conclusions are. Discussion After compend of the data, it is clear tha t our hypothesis was correct, because the found peak does fall within the range of 23 and 75.We can be fairly certain that our data are correct because we were able to control the fixed variables, such as the amounts of each component of the reaction, very well by having the same person measure out the same thing for each trial. We were fairly accurate at controlling the temperature because the baths were monitored at a steady temperature for the time the reaction was taking place, although it would have probably been to our receipts to actually take the temperature of the liquids inside the test tubes instead of only monitoring the environment outside of the tubes.Also, we could have increased accuracy by having the spectrophotometer nearer to the baths, because at that place was some heat lost during transport, which could have affected the tubes that were measured later. There was in like manner some confusion at the beginning of the experiment about exactly what technique was to be used, which resulted in our having to repeat the trials for the 65-degree temperature. We assume that similar problems are to blame for the discrepancys in the reaction rates for 37o and 45o between our group and group A-4.We excessively discovered after the experiment had been completed that the other group had left the catechol out in a separate tube during heating, while we left the enzyme out. This difference in method may have contributed to the differences. To make sure that these problems did not result in faulty conclusions, I looked up another experiment on the effects of temperature done by Pierre Greenway. Greenways findings indicate that the peak temperature for enzyme function is actually at 40oC, and not 45.This is an interesting discovery, since we did not test thoroughly in the range of 37o-45o. The next course of experimentation suggested by these findings would be to thoroughly test the reaction rates at temperatures between 35 and 45 degrees Celsius. mea surement at one-degree intervals would give the best results, but may require another collaboration to be achieved in a reasonable amount of time. It may also be interesting to test the reaction rate at even higher temperatures, and try to find the point at which all of the enzyme is completely destroyed.According to the results of Greenway, this temperature was around 60oC for him, but we tested beyond that in our own experiment and found the rate to still be decreasing. Any of these questions would be interesting to try to answer. Show The above preview is unformatted text This student written piece of work is one of many that can be found in our GCSE Patterns of conduct section.